An overview of proteomics and mass spectrometry workflows, experimental design, and data analysis.

Part I: Protein identification (peptide mass fingerprinting, tandem mass spectrometry, database searching, spectrum library searching, de novo sequencing, significance testing). (Slides, Video)

Part II: Protein characterization (protein coverage, top-down proteomics, post-translational modifications, protein processing and degradation, protein complexes). (Slides, Video)

Part III: Protein quantitation (metabolic labeling - SILAC, chemical labeling, label-free quantitation, spectrum counting, stoichiometry, biomarker discovery and verification) Examples will be provided throughout the course on how the different approaches can be applied to investigate biological systems. (Slides, Video)